Table 1

Percentage of positive immunostained Notch-1, Stro-1, VCAM-1, and Sox9 cells

Molecule

Zone

Percentage positive (±SE)


Normal

OA Non-fibrillated

OA Fibrillated

OA clusters


Notch-1

Superficial

71.5 ± 3.2†

57.7 ± 9.0*

84.2 ± 3.7†**#

83.6 ± 7.0 (1/5¶)

Normal (n = 8)Δ

OA NF (n = 5)

OA Fib (n = 5)

Middle

34.8 ± 6.7

48.9 ± 6.6*

61.6 ± 7.4†**

80.9 ± 7.2 (6/40)

Deep

29.1 ± 6.9

28.2 ± 10.6†

10.4 ± 5.6†**

68.68 ± 7.2 (2/11)

Mean: 78.5 ± 5.2


Stro-1

Superficial

81.3 ± 5.9†

84.7 ± 4.4†

82.9 ± 2.2

90.5 ± 9.5 (2/17)

Normal (n = 9)

OA NF (n = 8)

OA Fib (n = 4)

Middle

51.8 ± 7.6

56.8 ± 5.4†

71.2 ± 12.7

75.1 ± 14.7 (4/31)

Deep

38.3 ± 12.3

42.0 ± 8.2†

42.8 ± 20.6†

37.0 ± 9.15§ (2/11)

Mean: 69.4 ± 10.4


VCAM-1

Superficial

84.1 ± 1.3†

82.5 ± 4.7†

90.8 ± 5.5†

88.6 ± 7.7 (2/14)

Normal (n = 4)

OA NF (n = 6)

OA Fib (n = 4)

Middle

41.0 ± 8.9†

65.3 ± 4.4†*

66.5 ± 6.3**

76.5 ± 8.9 (3/24)

Deep

15.7 ± 5.5†

44.5 ± 6.8†*

60.7 ± 11.3**

61.1 ± 3.7 (3/19)

Mean: 75.4 ± 1.6


Sox9

Superficial

68.5 ± 6.9†

48.5 ± 8.8*

69.0 ± 5.3#

81.8 ± 5.2 (3/27)

Normal (n = 8)

OA NF (n = 8)

OA Fib (n = 6)

Middle

48.4 ± 6.1

43.2 ± 7.1

43.0 ± 8.6

73.5 ± 4.9 (4/30)

Deep

38.0 ± 9.3

39.5 ± 11.3

18.5 ± 11.7#

25.6 ± 11.4§ (4/27)

Mean: 71.6 ± 4.9


Results show percentage positive cells for each zone in normal, non-fibrillated (NF), fibrillated (Fib) osteoarthritic (OA), and cells in OA cell clusters in human articular cartilage (number of donors).

P < 0.05 between zone comparisons within each condition. * P < 0.05 between normal and non-fibrillated OA in the corresponding zone. ** P < 0.05 between normal and fibrillated OA in the corresponding zone. # P < 0.05 between non-fibrillated OA and fibrillated OA in the corresponding zone. ¶ (number of donors assessed/total number of clusters counted). § P < 0.05 between clusters in each zone within each condition. Δ Number of donors stained for each marker and condition.

SE = standard error; VCAM-1 = vascular cell adhesion molecule-1.

Grogan et al. Arthritis Research & Therapy 2009 11:R85   doi:10.1186/ar2719

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