Table 1 |
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Synthetic Toll-like receptor-9 agonists and antagonists used in the study |
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TLR9-antagonists |
Class |
Properties |
|
|
|
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|
4084-F |
CCT GGA TGG GAA |
B |
5' linear, shortest active |
|
INH-1 |
CCT GGA TGG GAA TTC CCA TCC AGG |
R |
5' palindrome |
|
INH-18 |
CCT GGA TGG GAA CTT ACC GCT GCA |
B |
5' linear |
|
INH-43 |
CCT GGA TAA AAA TTT TTA TCC AGG |
R |
Lacks GGG |
|
INH-44 |
CCT GAA TAA AAA CTT ACC GCT GCA |
B |
Lacks GGG |
|
INH-45 |
TAT GGA TGG GAA TTC CCA TCC ATA |
R |
Lacks CCT |
|
INH-46 |
TAT GGA TGG GAA CTT ACC GCT GCA |
B |
Lacks CCT |
|
INH-47 |
TAT GGA TTT TAA TTA AAA TCC ATA |
R |
Lacks CCT/GGG |
|
INH-48 |
TAT GGA TTT TAA CTT ACC GCG GCA |
B |
Lacks CCT/GGG |
|
5' OVHG-short |
CCT GGA TGG GAA TTC CCA TCC |
R |
5' short overhang (3 nucleotide OVHG) |
|
5' OVHG-scr. short |
CCT GGA TGG GAA CTT ACC GCT |
B |
5' linear |
|
5' OVHG-medium |
CCT GGA TGG GAA TTC CCA |
R |
5' medium overhang (6 nucleotide OVHG) |
|
5' OVHG-scr. medium |
CCT GGA TGG GAA CTT ACC |
B |
5' linear |
|
5' OVHG-long |
CCT GGA TGG GAA TTC |
R |
5' long overhang (9 nucleotide OVHG) |
|
5' OVHG-scr. long |
CCT GGA TGG GAA CTT |
B |
5' linear |
|
INH-4 3' palindrome |
TTC CCA TCC AGG CCT GGA TGG GAA |
R |
3' palindrome |
|
INH-13 3' scr. pal. |
CTT ACC GCT GCA CCT GGA TGG GAA |
B |
3' linear |
|
3' OVHG-short |
CCA TCC AGG CCT GGA TGG GAA |
R |
3' short overhang (3 nucleotide OVHG) |
|
3' OVHG-scr. short |
ACC GCT GCA CCT GGA TGG GAA |
B |
3' linear |
|
3' OVHG-medium |
TCC AGG CCT GGA TGG GAA |
R |
3' medium overhang (6 nucleotide OVHG) |
|
3' OVHG-scr. medium |
GCT GCA CCT GGA TGG GAA |
B |
3' linear |
|
3' OVHG-long |
AGG CCT GGA TGG GAA |
R |
3' long overhang (9 nucleotide OVHG) |
|
3' OVHG-scr. long |
GCA CCT GGA TGG GAA |
B |
3' linear |
|
4173 |
RRR RRR RRR RRR RRR |
B |
Linear control, R = random |
|
TLR9-agonists |
Class |
Properties |
|
|
CpG-2336 |
ggGGACGACGTCGTGgggggg |
A(D) |
Lowercase PS linkages |
|
CpG-1826 |
TCC ATG ACG TTC CTG ACG TT |
B(K) |
Linear, murine |
|
CpG-2006 |
TCG TCG TTT TGT CGT TTT GTC GTT |
B(K) |
Linear, human |
|
|
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OVHG, overhang; pal., palindrome; PS, phosphorothioate; scr., scrambled; TLR, Toll-like receptor. |
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Lenert et al. Arthritis Research & Therapy 2009 11:R79 doi:10.1186/ar2710 |
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