Research article
Differential gene expression in the salivary gland during development and onset of xerostomia in Sjögren's syndrome-like disease of the C57BL/6.NOD-Aec1Aec2 mouse
-
* Corresponding author: Ammon B Peck peck@pathology.ufl.edu
1 Department of Oral Biology, College of Dentistry, 1600 SW Archer Rd., University of Florida, Gainesville, FL 32610, USA
2 Center for Biotechnology & Genomic Medicine, CBGM 1120 15th Street CA4126, Medical College of Georgia, Augusta, GA 30912, USA
3 Department of Pathology, Immunology & Laboratory Medicine, College of Medicine, 1600 SW Archer Rd., University of Florida, Gainesville, FL 32610, USA
4 Center for Orphan Autoimmune Diseases, College of Dentistry, 1600 SW Archer Rd., University of Florida, Gainesville, FL 32610, USA
Arthritis Research & Therapy 2009, 11:R56 doi:10.1186/ar2676
Published: 20 April 2009Additional files
Additional file 1:
Verification of microarray data by RT-PCR. Four genes, Ctsb, Apoe, Akt1 and Fdft1, identified as displaying different levels of transcripts in the microarrays of salivary glands over time were selected for RT-PCR analyses. A. PCR band intensities visualized with ethidium bromide staining. B. Plot of PCR band intensities. C. Corresponding plot of transcripts as determined by microarray. G3pdh was used to control for fidelity of the RT-PCRs. As the band intensities of G3pdh remained constant over all the time points, the relative band intensities are not shown here.
Format: PPT Size: 194KB Download file
This file can be viewed with: Microsoft PowerPoint Viewer
