Figure 7.

mRNA expression of endothelial and inducible nitric oxide synthase in animals with collagen-induced arthritis. mRNA expression of (a) endothelial nitric oxide synthase (eNOS) and (b) inducible nitric oxide synthase (iNOS) in the inguinal lymph nodes of animals with CIA and either the normal diet (N) or the phosphatidylcholine-enriched diet, starting either with the CIA induction (PC_pre) or with the clinical onset of disease (PC_ther). For the induction of CIA, animals were immunized twice with collagen II and complete Freund's adjuvant/incomplete Freund's adjuvant. Three weeks after the second immunization, lymph nodes were taken from the inguinal region and prepared as described in Materials and methods. The expressions of the target genes were referred to that of GADPH. Values given as medians with the 25th and 75th percentiles. #P < 0.05 versus N/CIA. $P < 0.05 versus N/control.