Figure 3.

A representative two-dimensional differential gel electrophoresis (2D-DIGE) analysis of neutrophil gelatinase-associated lipocalin (NGAL)-affected proteins in synoviocytes. Proteins from synoviocytes treated with NGAL or untreated were labelled separately with Cy5 (green) and Cy3 (red), and then were separated on the same gel using the 2D-DIGE system. Approximately 3,600 protein spots were visualised by laser scanning. On treatment with NGAL, the intensities of 15 and 6 protein spots increased by up to more than 1.5-fold and decreased by less than 1 to 1.5-fold, respectively. Identified protein spots are indicated by open circles. The number near the circle is the spot number, as indicated in Table 4.