Figure 5.

Phenotypic characteristics of injected mesenchymal stem cells following 4 weeks in culture. Photomicrographs representative of 4',6-diamidino-2-phenylindole (DAPI) counterstaining, green fluorescent protein (GFP)localisation and immunofluorescence (IF) for identical field of view and immunohistochemistry (IHC) in tissue injected with mesenchymal stem cells and cultured for 4 weeks post injection: (a) Sox-9, (b) aggrecan (Agg) and (c) type II collagen (Coll II). High Mag IHC, high magnification of indicated IHC region. Scale bar = 570 μm.