Figure 1.

Lipocalin-type prostaglandin D synthase (L-PGDS) and hematopoietic-type PGDS (H-PGDS) mRNA levels in healthy and osteoarthritis (OA) human cartilage. RNA was extracted from healthy (n = 9) and OA (n = 9) cartilage, reverse-transcribed into cDNA, and processed for real-time polymerase chain reaction. The threshold cycle values were converted to the number of molecules, as described in Materials and methods. Data are expressed as copies of the gene's mRNA detected per 10,000 GAPDH copies. *P < 0.05 versus healthy samples. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.