Figure 6.

Titres of anti-mouse glucose-6-phosphate isomerase (GPI) antibodies were elevated in mice with arthritis. (a) Sera were taken on day 14 from mice immunised with recombinant human (rh) GPI, hGPI_325–339, hGPI_544–558 or hGPI_325–339 plus hGPI_544–558, and the titres of anti-human GPI antibodies and anti-mouse GPI antibodies were analysed by ELISA. Each symbol represents a single mouse. Data are mean optimal density ± standard deviation. †p < 0.01 (Mann-Whitney's U test). Representative data of two independent experiments. (b) Ankle joints were taken on day 14 from mice immunised with rhGPI, hGPI_325–339 or hGPI_544–558. Cryostat sections of ankle joints were stained with anti-mouse IgG (red), and nuclei were counterstained with 4',6-diamidino-2-phenyindole dilactate (blue). Representative data of three independent experiments.