Characterisation of antibody responses in collagen-induced arthritis (CIA) and experimental autoimmune encephalomyelitis (EAE) using myelin and synovial antigen arrays. (a, b) Synovial and (d, e) myelin arrays were produced by printing synovial and myelin peptides and proteins in ordered arrays on the surface of microscope slides. The arrays were probed with 1:150 dilutions of (a, d) normal, (b) CIA and (e) EAE sera followed by a cyanine 3 labelled anti-mouse IgG/M secondary antibody. Scanned images of the slides are presented in false colour representation. Yellow features represent marker spots to orient the array. Green features represent serum antibody reactivity. Reactive features are highlighted and quantitative values are presented in panels c and f.
Kidd et al. Arthritis Research & Therapy 2008 10:R119 doi:10.1186/ar2523