Figure 1.

4-Hydroxynonenal (HNE)-induced cell death. (a) Chondrocytes were pre-incubated for 1 hour with or without 200 μM N-acetyl-cysteine (NAC) followed by another incubation for 16 hours with increasing concentrations of HNE (0 to 30 μM). Chondrocytes were pre-incubated for 1 hour with or without (b) 20 μg/mL anti-Fas/CD95 antibody or (c) 50 and 100 μM INH₂BP followed by another incubation for 16 hours with or without 30 μM HNE. Cell viability was evaluated by MTT assay. Data are mean ± standard error of the mean (n = 8). Statistics: Student unpaired t test; ***P < 0.001 (30 μM HNE versus untreated cells), ^#P < 0.05 (30 μM HNE+inhibitor versus 30 μM HNE).