Table 1 |
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Genes studied with QPCR for confirmatory studies, primers and probe sequences |
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| Accession number |
Gene symbol |
Target exonb |
Probe |
Forward primer |
Reverse primer |
|
|
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| Up-regulated in DA |
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| NM_139089.1 |
Cxcl10 |
4 |
Exiqon Universal probe 67 |
TTCGGACCAGCTCTTAGAGAA |
GCCTGGTCCTGAGACAAAAG |
| XM_220552.3 |
Trim16 |
6 |
Exiqon Universal probe 1 |
GTGAACTCCTTCCCACTCCA |
CAGCTGCATTTCTGGAAACA |
| NM_017207.1 |
Trpv2 |
15 |
Exiqon Universal probe 6 |
CTCTTCCCACCTTATCTGAGGA |
GACCTGAAGGGGCAGATG |
| NM_019357.1 |
Vil2 |
13 |
CCCCAAGACCCAGTGGAATCCTCCa |
AGGTACCGGGCGATGTTCT |
GGCCTGTTTGGCACTATGTGA |
| LOC309362 |
Dnmbp |
16 |
Exiqon Universal probe 97 |
TTGTCTCAGCATGGGTCCTA |
ACCAGGATTTTAAGGCCACA |
| NM_001107408 |
Gins3 |
3–4 |
Exiqon Universal probe 17 |
GTCGTGGACCTCCACAAAAT |
GAACCGTCCAATAAAAGTCTGC |
| Down-regulated in DA |
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| XM_235434.4 |
Gsdmdc1 |
13 |
Exiqon Universal probe 68 |
AGCACGTCTTGGAACAGAGC |
TCCTCATCCCAGCTGTCC |
| XM_222868.4 |
Olfml2b |
8 |
Exiqon Universal probe 106 |
CTCCCTTCTTCCATGCTCTG |
GCAAGCCCCAGAGGAATAA |
| NM_001008321.1 |
Gadd45b |
4 |
Exiqon Universal probe 25 |
ACAGGTGGTCGCCAAGAC |
CCAGGCCTTGGCTCTAAAGT |
| Estrogen receptors |
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| NM_012689.1 |
Esr1 |
- |
Exiqon Universal probe 67 |
GCAAGAATGTCGTGCCTCTC |
TGAAGACGATGAGCATCCAG |
| NM_012754 |
Esr2 |
- |
Exiqon Universal probe 94 |
CCTTGAAGGCTCTCGGTGTA |
CAGAACCTTTCAGATGTTTCCA |
|
|
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|
aTaqman probe. bSame region used in the Illumina microarray. |
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|
Laragione et al. Arthritis Research & Therapy 2008 10:R92 doi:10.1186/ar2476 |
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