Table 1 |
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|
Profile of antibodies used in 3D immunohistological and CD marker studies |
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|
Antibody |
Source |
Dilution |
|
|
||
|
Type I collagen |
Biodesign International (Kennebunk, ME) |
20 μg/ml |
|
Type II collagen |
Biodesign International (Kennebunk, ME) |
20 μg/ml |
|
Decorin |
R&D Systems, (Minneapolis, MN) |
25 μg/ml |
|
Keratin sulphate |
Seikagaku Corporation, (Tokyo, Japan) |
5 μg/ml |
|
Chondroitin sulphate |
ICN Biomedicals, (Costa Mesa, CA) |
20 μg/ml |
|
CD29 |
Lab Vision Corporation (Fremont, CA) |
200 μg/ml |
|
CD34 |
DakoCytomation (Carpinteria, CA) |
50 μg/ml |
|
CD45 |
DakoCytomation, (Carpinteria, CA) |
350 μg/ml |
|
CD105 |
Lab Vision Corporation (Fremont, CA) |
200 μg/ml |
|
|
||
|
3D, three dimensional; CD, cluster of differentiation. |
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|
Tapp et al. Arthritis Research & Therapy 2008 10:R89 doi:10.1186/ar2473 |
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