Osteogenic differentiation of adipose-derived mesenchymal stem cells (AD-MSC). (a) and (b) AD-MSC treated with osteogenic media for three weeks stained with Alizarin red. Red staining marks mineralised matrix produced by osteoblasts. A at × 4 magnification; (b) at × 105 magnification. (c) High density cultures showed formation of a chondrogenic phenotype when cultured in micromass; pink extracellular matrix staining marks proteoglycans stained with toluidine blue × 95.
Tapp et al. Arthritis Research & Therapy 2008 10:R89 doi:10.1186/ar2473