Table 1

Details of polymerase chain reaction (PCR) primers, probes, and amplicon sizes

Conventional PCR conditions


Target

Forward primer 5' to 3'

Reverse primer 5' to 3'

Amplicon size, base pairs (bp)

18S

GCC ATG CAT GTC TAA GTA CG

GCT GGC ACC AGA CTT GCC

574 bp

Caveolin-1

AAG GAG ATC GAC CTG G

GGA ATA GAC ACG GCT G

309 bp


Real-time PCR primers and probes


Target

Forward primer 5' to 3'

Probe 5' to 3'

Reverse primer 5' to 3'

18S

PDAR

PDAR (VIC-TAMRA)

PDAR

Caveolin-1

ACT TGC AAC CGT CTG TTA TGC T

FAM – ACA TGG CCC CTC CCC – MGB

GCA AAG GGA TGC TTG GAT TAG GT

p16INK4a

GGC TCT ACA CAA GCT TCC TTT CC

FAM – ACC CTG GCT CTG ACC A – MGB

TCA TGA CCT GCC AGA GAG AAC A


PDAR, pre-developed assay reagent.

Heathfield et al. Arthritis Research & Therapy 2008 10:R87   doi:10.1186/ar2468

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