Quantitative real-time reverse transcription-polymerase chain reaction analysis of caveolin-1 gene expression levels in nucleus pulposus (NP) cells from human intervertebral disc. (a) Caveolin-1 gene expression per 100 ng cDNA normalised to 18S in non-degenerate and degenerate NP presented as box-and-whisker plot (5–95 percentile). (b) Correlation of caveolin-1 gene expression with age of subject. Non-parametric linear regression analysis (P = 0.0472; R2 = 0.2122).
Heathfield et al. Arthritis Research & Therapy 2008 10:R87 doi:10.1186/ar2468