Conventional reverse transcription-polymerase chain reaction (RT-PCR) for caveolin-1 and housekeeping gene 18S. Representative photographs following agarose gel electrophoresis of products from conventional RT-PCR for caveolin-1 and 18S. cDNA samples displayed are non-degenerate samples from a post mortem (PM) source (respective grades [G] and ages of subjects: G3, 30 years; G1, 30 years; and G2, 75 years) and degenerate samples from surgical and PM sources (G5, 29 years; G6, 34 years; and G9, 74 years). Photographs are inverted to improve visualisation of product bands. Cav-1, caveolin-1.
Heathfield et al. Arthritis Research & Therapy 2008 10:R87 doi:10.1186/ar2468