Table 1

Reverse transcription-polymerase chain reaction primers for differentiation-specific gene expression analysis

Genes

Primer sequences (5'-3')

Position, base pairs

Predicted size, base pairs

Housekeeping gene

GAPDH

Sense: GGACTCATGACCACAGTCCATGCC

619–770

152

Antisense: TCAGGGATGACCTTGCCCACA

Bone-specific genes

ALP

Sense: TGGAGCTTCAGAAGCTCAACACCA

379–832

454

Antisense: ATCTCGTTGTCTGAGTACCAGTCC

OC

Sense: ATGAGAGCCCTCACACTCCTC

19–312

294

Antisense: GCCGTAGAAGCGCCGATAGGC

Adipose-specific genes

LPL

Sense: GAGATTTCTCTGTATGGCACC

1,457–1,732

276

Antisense: CTGCAAATGAGACACTTTCTC

PPARγ

Sense: TGAATGTGAAGCCCATTGAA

1,476–1,636

161

Antisense: CTGCAGTAGCTGCACGTGTT

Cartilage-specific genes

AGN

Sense: TGCGGGTCAACAGTGCCTATC

655–836

182

Antisense: CACGATGCCTTTCACCACGAC

COL2A1

Sense: GGAAACTTTGCTGCCCAGATG

710–876

167

Antisense: TCACCAGGTTCACCAGGATTGC

AGN, aggrecan; ALP, alkaline phosphatase; COL2A1, collagen type II α1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LPL, lipoprotein lipase; OC, osteocalcin; PPARγ, peroxisome proliferator-activated receptor-gamma.

Janjanin et al. Arthritis Research & Therapy 2008 10:R83   doi:10.1186/ar2459