Cell surface characterisation of infrapatellar fat pad (IPFP) cells. Cell surface staining on passage 2 IPFP cells expanded in the absence (a) and presence (b) of fibroblast growth factor-2 (FGF-2) was performed using a panel of antibodies and fluorescein isothiocyanate-conjugated secondary antibody (green) and DAPI (4'-6-diamidino-2-phenylindole) (blue). Results showed strong staining for CD13, CD29, CD44, CD90, and CD105, weak staining for 3G5, and negative staining for LNGFR, STRO1, CD34, CD56, and the IgG control. The FGF-2-expanded cells are morphologically different from cells expanded in the absence of FGF-2 but show a similar cell surface expression.
Khan et al. Arthritis Research & Therapy 2008 10:R74 doi:10.1186/ar2448