Figure 2.

Association of HLA-B27 with TAP in KG-1 cells. KG-1.B27sv5 and KG-1.B27.H114D.D116Ysv5 cells were stimulated for 24 hours, lysed in digitonin-containing buffer and immunoprecipitated with anti-TAP1 antibodies conjugated to Protein G beads. Samples and cell lysate controls were resolved on sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted with the indicated antibodies. Molecular mass markers are shown in kDa.