Table 1

Production of IL-6 following 24 hours and 48 hours of stimulation with different doses of purified recombinant endotoxin-free high mobility group box chromosomal protein 1 (pHMGB1) and mouse recombinant high mobility group box chromosomal protein 1 (rHMGB1)
Time
 Mice
 IL-6 production (pg/ml)
 Lipopolysaccharidea





0 μg/ml HMGB1
 0.05 μg/ml HMGB1
 0.5 μg/ml HMGB1
 5 μg/ml HMGB1
PHMGB1





     24 hours
 TNFα-/-
 17.0 ± 2.3
 17.6 ± 2.2
 17.8 ± 2.4
 13.1 ± 4.6
 2,460 ± 226

TNFα+/+
 16.2 ± 1.9
 15.9 ± 2.0
 16.8 ± 3.2
 7.3 ± 3.1
 6,905 ± 1,763
     48 hours
 TNFα-/-
 19.1 ± 3.0
 20.1 ± 2.9
 17.0 ± 1.6
 26.3 ± 3.8

TNFα+/+
 19.8 ± 2.2
 18.3 ± 2.2
 14.2 ± 2.3
 20.2 ± 3.2
RHMGB1





     24 hours
 TNFα-/-
 28 ± 16
304 ± 50* (314)
 2,317 ± 264 (2,271)
 146 ± 46* (130)

TNFα+/+
 68 ± 11
602 ± 112 (558)
 3,046 ± 757 (2,504)
 344 ± 63 (359)
     48 hours
 TNFα-/-
 213 ± 58
468 ± 56* (500)
 2,541 ± 258 (2,588)
 238 ± 79 (207)

TNFα+/+
 263 ± 33
1,133 ± 202 (1,062)
 4,114 ± 941 (3,527)
 438 ± 62 (408)

Data presented as the mean ± standard error of the mean of 10 mice per each group (pHMGB1), and the mean ± standard error of the mean (median) of four mice in each group (rHMGB1). HMGB1, high mobility group box chromosomal protein 1. apHMGB1, 5 μg/ml; rHMGB1, 10 ng/ml. *P < 0.05 TNFα-/- mice versus control mice.

Pullerits et al. Arthritis Research & Therapy 2008 10:R72   doi:10.1186/ar2445