Table 1 |
||||
|
Primers used for RT-PCR |
||||
| Gene name |
Primer sequence |
Product length |
Annealing temperature |
Number of cycles |
|
|
||||
| BMP-2 |
Forward: GCT CTT TCA ATG GAC GTG TC Reverse: GCT CTG CTG AGG TGA TAA AC |
514 bp |
59°C |
38 |
| Chordin |
Forward: GAG AAC TTC AGG CCA ATG TC Reverse: CAG TGG GTA TCC AAG GAA AG |
654 bp |
58°C |
40 |
| GAPDH |
Forward: CCA TCA CCA TCT TCC AGG AG Reverse: CAT CCA CAG TCT TCT GGG TG |
353 bp |
60°C |
32 |
|
|
||||
|
bp, base pairs; BMP, bone morphogenetic protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. |
||||
|
Kwong et al. Arthritis Research & Therapy 2008 10:R65 doi:10.1186/ar2436 |
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