|
Validation of array data by real-time quantitative RT-PCR |
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| Gene |
Probe set |
Correlation coefficient |
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|
|
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| U133A |
U95A |
U133A versus RT-PCR (n = 19) |
U133A versus U95A (n = 11) |
U95A versus RT-PCR (n = 11) |
|
|
|
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| ICAM1 |
202637_s_at |
32640_at |
0.9329 |
0.8916 |
0.8560 |
| TNFAIP3 |
202643_s_at |
595_at |
0.9437 |
0.9537 |
0.9792 |
| IL1B |
39402_at |
39402_at |
0.9443 |
0.9623 |
0.9667 |
| PDE4B |
211302_s_at |
33705_at |
0.8880 |
0.9583 |
0.6307 |
| PPP1R15A |
37028_at |
37028_at |
0.9519 |
0.9869 |
0.7649 |
|
Pearson correlation coefficients between real-time quantitative RT-PCR data (-ΔΔCT t1 versus t0) and the microarray data from the GeneChip U133A and U95A for five selected genes found to be differentially regulated in responders and nonresponders are presented. | |||||
Koczan et al. Arthritis Research & Therapy 2008 10:R50 doi:10.1186/ar2419 |
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