Figure 2.

Fibroblast growth factor receptor 1 expression is upregulated in normal bovine nucleus pulposus tissue. Nucleus pulposus cells isolated from bovine intervertebral disc were cultured in a monolayer in 12-well plates at 8 × 10⁵ cells/cm² for 48 hours and the total RNA was extracted to perform real-time RT-PCR of fibroblast growth factor receptor (FGFR1, FGFR2, FGFR3 and FGFR4) genes. Error bars represent three different donors in three separate experiments.