Coculture with stimulated rheumatoid arthritis synovial fibroblasts doubles the functional lifespan of peripheral blood neutrophils. (a) Peripheral blood neutrophils were cocultured with untreated rheumatoid arthritis synovial fibroblasts (RASF) (open squares) or with RASF stimulated with IL-17 and TNFα (RASFIL-17/TNF) (closed squares), and their survival was assessed every 24 hours by flow cytometry. ***P < 0.001. (b) The ability to produce superoxide radical in response to f-Met-Leu-Phe was determined in freshly isolated neutrophils (open bars) and neutrophils cocultured with RASFIL-17/TNF for 24 hours (filled bars). **P < 0.01 versus unstimulated cells.
Parsonage et al. Arthritis Research & Therapy 2008 10:R47 doi:10.1186/ar2406