Research article
The influence of serum, glucose and oxygen on intervertebral disc cell growth in vitro: implications for degenerative disc disease
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* Corresponding author: William EB Johnson Eustace.Johnson@rjah.nhs.uk
1 Centre for Spinal Studies, Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry, Shropshire, SY10 7AG, UK
2 Institute for Science and Technology in Medicine, Keele University, Keele, Staffordshire, ST5 5BG, UK
Arthritis Research & Therapy 2008, 10:R46 doi:10.1186/ar2405
Published: 23 April 2008Additional files
Additional file 1:
'Live/dead' staining of intervertebral disc (IVD) cells in alginate cultures. A representative image is shown of IVD cells in alginate cultured in serum-deprived conditions for 7 days; the pycnotic nucleus of a nonviable cell appears bright red, with viable cells appearing green (original magnification ×400).
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