The influence of serum, glucose and oxygen on intervertebral disc cell growth in vitro: implications for degenerative disc disease

William EB Johnson¹^,2 , Simon Stephan¹^,2 and Sally Roberts¹^,2

¹Centre for Spinal Studies, Robert Jones and Agnes Hunt Orthopaedic Hospital, Oswestry, Shropshire, SY10 7AG, UK

²Institute for Science and Technology in Medicine, Keele University, Keele, Staffordshire, ST5 5BG, UK

author email corresponding author email

Arthritis Research & Therapy 2008, 10:R46doi:10.1186/ar2405


Published:	23 April 2008

Additional files

Additional file 1:

'Live/dead' staining of intervertebral disc (IVD) cells in alginate cultures. A representative image is shown of IVD cells in alginate cultured in serum-deprived conditions for 7 days; the pycnotic nucleus of a nonviable cell appears bright red, with viable cells appearing green (original magnification ×400).

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