Footpad histology and immunohistochemistry. Samples were obtained 24 hours after soluble tissue factor injection. (a) Wildtype mice showed marked inflammatory changes. (b) Phosphate-buffered saline-injected mice showed minimal signs of inflammation. (c) Staining for macrophages was strongly positive. (d) CD3-positive T cells were also present. (e) Staining specificity was confirmed using, as primary antibody, nonimmune isotype-matched antibodies. (f) Fibrin deposition was assessed by fibrin immunohistochemistry.
Busso et al. Arthritis Research & Therapy 2008 10:R42 doi:10.1186/ar2400