No changes in TNF-induced osteoclast precursor release from Cxcr4 f/f/CD11b+/Cre conditional knockout mice bone marrow. (a) Bone marrow cells from 2-month-old CXCR4 CKO and control mice were double stained with FITC-anti-CD11b and allophycocyanin (APC)–anti-CXCR4 antibodies, and CD11b+ cells were gated. Intensity of CXCR4+ stained CD11b+ cells shown. Black line, CXCR4 CKO cells; red line, control cells. (b) Murine TNF (0.5 μg/injection, 4 times/day × 3 days, intraperitoneally) or PBS was injected into 2-month-old CXCR4 CKO mice and control mice (n = 2 per group). Two hours after the last TNF injection, the peripheral blood was harvested and double stained with APC–anti-CD11b and Phycoerythrin-anti-Gr1 antibodies. The percentage of CD11b+/Gr-1-/lo osteoclast precursors (OCPs) were determined. Values from individual mice are plotted. bp, base pairs; WT, wild-type.
Zhang et al. Arthritis Research & Therapy 2008 10:R37 doi:10.1186/ar2391