Decreased stromal cell-derived factor-1 expression in bone marrow stromal cells and bones of TNF-transgenic mice. (a) Bone marrow stromal cells from 6-month-old TNF-transgenic (TNF-Tg) mice and wild-type (WT) littermates were cultured in α-modified essential medium plus 20% fetal bovine serum for 7 days. The stromal cell-derived factor 1 (SDF-1) protein concentration in the conditioned medium was assessed by ELISA (upper panel). Expression levels of SDF-1 mRNA were determined by real-time RT-PCR (lower panel). Fold changes were calculated using the value from WT mice as 1. (b) Long bones from the above mice were harvested and subjected to RNA extraction. Expression of SDF-1 was measured by real-time RT-PCR. Data are the mean ± standard error of the mean of three loadings. The same results were obtained from three pairs of TNF-Tg mice and WT littermates. *P < 0.05 versus samples from WT littermates.
Zhang et al. Arthritis Research & Therapy 2008 10:R37 doi:10.1186/ar2391