Table 2

Primers used for real-time PCR and product length

Gene encoding

Primer sequence

Size (base pairs)

T_m (°C)

RP29

Sense: 5'-AAGATGGGTCACCAGCAGCTCTACTG-3'

67

59

Antisense: 5'-AGACGCGGCAAGAGCGAGAA-3'

Aggrecan

Sense: 5'-ACACCCCTACCCTTGCTTCT-3'

124

58

Antisense: 5'-AAAGTGTCCAAGGCATCCAC-3'

PPAR-α

Sense: 5'-GATGACCTGGAAAGTCCCTT-3'

59

56

Antisense: 5'-CTTGAATGTTTCCCATCTCTT-3'

PPAR-γ

Sense: 5'-ATGGGTGAAACTCTGGGAGAT-3'

92

56

Antisense: 5'-GGTAATTTCTTGTGAAGTGCT-3'

Adiponectin

Sense: 5'-AATCCTGCCCAGTCATGAAG-3'

433

58

Antisense: 5'-TCTCCAGGAGTGCCATCTCT-3'

ACO

Sense: 5'-CCAATCACGCAATAGTTCTGG-3'

362

57

Antisense: 5'-CGCTGTATCGTATGGCGAT-3'

PPAR, peroxisome proliferator-activated receptor; ACO, acyl-CoenzymeA oxidase; T_m, melting temperature.

Koufany et al. Arthritis Research & Therapy 2008 10:R6   doi:10.1186/ar2354