Table 1

Primers used for semi-quantitative PCR and product length

Gene encoding

Primer sequence

Size (base pairs)

Cycles

Tm (°C)


L27

Sense: 5'-TCCTGGCTGGACGCTACTC-3'

225

27

62

Antisense: 5'-CCACAGAGTACCTTGTGGGC-3'

MCP-1

Sense: 5'-ATGCAGTTAATGCCCCACTC-3'

167

29

57

Antisense: 5'-TTCCTTATTGGGGTCAGCAC-3'

bFGF

Sense: 5'-GAACCGGTACCTGGCTATGA-3'

182

31

61

Antisense: 5'-CCGTTTTGGATCCGAGTTTA-3'

VEGF

Sense: 5'-CAATGATGAAGCCCTGGAGT-3'

211

32

64

Antisense: 5'-TTTCTTGCGCTTTCGTTTTT-3'

TNF-α

Sense: 5'-AGATGTGGAACTGGCAGAGG-3'

178

31

58

Antisense: 5'-CCCATTTGGGAACTTCTCCT-3'

IL-1β

Sense: 5'-TGAAAGCTCTCCACCTCAATGG-3'

366

28

61

Antisense: 5'-TCCATGGTGAAGTCAACTATGTCC-3'


MCP-1, monocyte chemotactic protein-1; bFGF, basic fibroblast growth factor; VEGF, vascular endothelial growth factor; Tm, melting temperature.

Koufany et al. Arthritis Research & Therapy 2008 10:R6   doi:10.1186/ar2354

Open Data