Table 1 |
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|
Primers used for semi-quantitative PCR and product length |
||||
|
Gene encoding |
Primer sequence |
Size (base pairs) |
Cycles |
Tm (°C) |
|
|
||||
|
L27 |
Sense: 5'-TCCTGGCTGGACGCTACTC-3' |
225 |
27 |
62 |
|
Antisense: 5'-CCACAGAGTACCTTGTGGGC-3' |
||||
|
MCP-1 |
Sense: 5'-ATGCAGTTAATGCCCCACTC-3' |
167 |
29 |
57 |
|
Antisense: 5'-TTCCTTATTGGGGTCAGCAC-3' |
||||
|
bFGF |
Sense: 5'-GAACCGGTACCTGGCTATGA-3' |
182 |
31 |
61 |
|
Antisense: 5'-CCGTTTTGGATCCGAGTTTA-3' |
||||
|
VEGF |
Sense: 5'-CAATGATGAAGCCCTGGAGT-3' |
211 |
32 |
64 |
|
Antisense: 5'-TTTCTTGCGCTTTCGTTTTT-3' |
||||
|
TNF-α |
Sense: 5'-AGATGTGGAACTGGCAGAGG-3' |
178 |
31 |
58 |
|
Antisense: 5'-CCCATTTGGGAACTTCTCCT-3' |
||||
|
IL-1β |
Sense: 5'-TGAAAGCTCTCCACCTCAATGG-3' |
366 |
28 |
61 |
|
Antisense: 5'-TCCATGGTGAAGTCAACTATGTCC-3' |
||||
|
|
||||
|
MCP-1, monocyte chemotactic protein-1; bFGF, basic fibroblast growth factor; VEGF, vascular endothelial growth factor; Tm, melting temperature. |
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|
Koufany et al. Arthritis Research & Therapy 2008 10:R6 doi:10.1186/ar2354 |
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