Table 2

Effect of CD47/IAP transfection on the electrophysiological response of CD47-null cells to mechanical stimulation

Membrane potential (-mV) (mean ± SEM)


Frequency of stimulation

Cell (phenotype)

Resting

Post mechanical stimulation

Percentage change


0.33 Hz

3657L (CD47 wild-type+)

10.9 ± 0.27

4.5 ± 0.64

-59a

3656 1/Sra (CD47-null)

14.0 ± 1.36

13.8 ± 0.46

-1b

3656 1/171 (mouse CD47 type 2+)

13.1 ± 0.52

5.9 ± 0.65

-55a

3656 1/315 (human CD47 type 2+)

11.8 ± 1.03

4.6 ± 1.03

-61a

0.083 Hz

3657L (CD47 wild-type+)

10.8 ± 0.6

11.9 ± 0.72

+10b

3656 1/Sra (CD47-null)

12.4 ± 0.43

13.0 ± 0.27

+5b

3656 1/171 (mouse CD47 type 2+)

12.8 ± 0.53

20.4 ± 0.92

+48a

3656 1/315 (human CD47 type 2+)

12.2 ± 0.91

17.2 ± 0.32

+42a

OV10 58A2 (CD47-null)

10.2 ± 0.85

10.8 ± 1.50

+6b

OV10 315 (human CD47 type 2+)

7.0 ± 0.84

18.6 ± 1.86

+165a

OV10 164/315 (β3/CD47 type 2+)

35.4 ± 1.6

49.2 ± 3.34

+39a

OV10 148A10T (CD47IgV/GPI)

22.6 ± 1.33

22.2 ± 1.07

0b


CD47-null cells or cells transfected with either type 2 CD47 cDNA, CD47 extracellular immunoglobulin variable (IgV) domain linked to a glycosylphosphatidylinositol anchor, or vector control were studied. Membrane potentials of cells were measured at rest and after 20 minutes of mechanical stimulation at 0.33 or 0.083 Hz. N = 5. ap ≤ 0.01; bnot significant; SEM, standard error of the mean.

Orazizadeh et al. Arthritis Research & Therapy 2008 10:R4   doi:10.1186/ar2350

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