Table 1

Effect of anti-CD47/IAP, anti-TSP-1, and anti-SIRPα on mechanically induced changes in human articular chondrocyte membrane potential

Membrane potential (-mV) (mean ± SEM)


Sample

Reagent

Resting

Reagent alone

Reagent + 0.33 Hz

Percentage change


Normal chondrocytes

Nil

25.0 ± 0.4

-

30.2 ± 0.6

+21a

Bric 126

26.2 ± 0.8

27.2 ± 0.4

27.6 ± 1.2

+2b

B6H12

25.8 ± 2.2

26.3 ± 1.9

17.1 ± 1.0

-35a

Osteoarthritic chondrocytes

Nil

30.0 ± 1.9

-

17.4 ± 1.3

-42a

Bric 126

28.0 ± 1.6

28.0 ± 2.1

27.4 ± 1.8

-2b

Anti-TSP-1

29.6 ± 1.2

29.0 ± 0.8

29.8 ± 1.6

+3b

SE5A5

29.2 ± 1.0

29.0 ± 1.1

23.2 ± 0.8

-20a


Membrane potentials of chondrocytes were measured at rest in the absence or presence of the appropriate antibody and after 20 minutes of mechanical stimulation at 0.33 Hz. Bric 126 is a function-blocking anti-CD47/IAP antibody. B6H12 is an anti-CD47/IAP antibody that has partial agonist activity. SE5A5 is an anti-SIRPα antibody. N = 5. ap < 0.001; bnot significant. IAP, integrin-associated protein; SEM, standard error of the mean; SIRPα, signal-regulatory protein-alpha; TSP-1, thrombospondin-1.

Orazizadeh et al. Arthritis Research & Therapy 2008 10:R4   doi:10.1186/ar2350

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