Table 1

Effect of anti-CD47/IAP, anti-TSP-1, and anti-SIRPα on mechanically induced changes in human articular chondrocyte membrane potential

Membrane potential (-mV) (mean ± SEM)

Sample

Reagent

Resting

Reagent alone

Reagent + 0.33 Hz

Percentage change

Normal chondrocytes

Nil

25.0 ± 0.4

-

30.2 ± 0.6

+21^a

Bric 126

26.2 ± 0.8

27.2 ± 0.4

27.6 ± 1.2

+2^b

B6H12

25.8 ± 2.2

26.3 ± 1.9

17.1 ± 1.0

-35^a

Osteoarthritic chondrocytes

Nil

30.0 ± 1.9

-

17.4 ± 1.3

-42^a

Bric 126

28.0 ± 1.6

28.0 ± 2.1

27.4 ± 1.8

-2^b

Anti-TSP-1

29.6 ± 1.2

29.0 ± 0.8

29.8 ± 1.6

+3^b

SE5A5

29.2 ± 1.0

29.0 ± 1.1

23.2 ± 0.8

-20^a

Membrane potentials of chondrocytes were measured at rest in the absence or presence of the appropriate antibody and after 20 minutes of mechanical stimulation at 0.33 Hz. Bric 126 is a function-blocking anti-CD47/IAP antibody. B6H12 is an anti-CD47/IAP antibody that has partial agonist activity. SE5A5 is an anti-SIRPα antibody. N = 5. ^ap < 0.001; ^bnot significant. IAP, integrin-associated protein; SEM, standard error of the mean; SIRPα, signal-regulatory protein-alpha; TSP-1, thrombospondin-1.

Orazizadeh et al. Arthritis Research & Therapy 2008 10:R4   doi:10.1186/ar2350