Expression of CD47/IAP by human articular chondrocytes. (a) Western blotting of protein extracts from human articular chondrocytes with three different anti-CD47/IAP antibodies (monoclonal antibodies 2B7, miap 400.1, and a goat polyclonal anti-CD47/IAP) showing a predominant band of approximately 50 kDa in protein extracts from chondrocytes extracted from both normal and osteoarthritic chondrocytes. (b) Reverse transcription-polymerase chain reaction on RNA extracted from primary cultures of chondrocytes from normal and osteoarthritic chondrocytes undertaken with specific primers for CD47/IAP showing predominant expression of the type 2 isoform. IAP, integrin-associated protein; OA, osteoarthritic human articular chondrocytes; OV10-315, CD47 negative ovarian carcinoma cell line transfected with human CD47 type 2 isoform; N, normal human articular chondrocytes; Neg, negative control (reverse transcription step omitted).
Orazizadeh et al. Arthritis Research & Therapy 2008 10:R4 doi:10.1186/ar2350