Rutoside (RU) mediates the inhibition of inflammatory gene transcription in activated human macrophages. Human monocyte-derived macrophages were activated (10 μg/mL CD23-McAb) alone or in the presence of 100 μM RU. After 24 hours of incubation, cellular mRNAs were extracted and analyzed by inflammation-specific macroarray. (a) RU inhibits gene expression by activated human macrophages, with the exception of interleukin (IL)-10. A representative array from two distinct experiments is shown. (b) Inflammatory genes detected on each membrane, in addition to controls. MIF, macrophage migration inhibitory factor; TNF, tumor necrosis factor.
Kauss et al. Arthritis Research & Therapy 2008 10:R19 doi:10.1186/ar2372