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Open Access Highly Accessed Research article

Rutoside decreases human macrophage-derived inflammatory mediators and improves clinical signs in adjuvant-induced arthritis

Tina Kauss12, Daniel Moynet1, Jérôme Rambert1, Abir Al-Kharrat1, Stephane Brajot1, Denis Thiolat1, Rachid Ennemany3, Fawaz Fawaz2 and M Djavad Mossalayi1*

Author Affiliations

1 Department of Immunology and Parasitology, EA3677, School of Pharmacy, Bordeaux 2 University, 146 rue Léo Saignat, 33076 Bordeaux, France

2 Department of Galenic and Biopharmaceutics, EA3677, School of Pharmacy, Bordeaux 2 University, 146 rue Léo Saignat, 33076 Bordeaux, France

3 Eurotest, 147 avenue de la Somme, 33700 Merignac, France

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Arthritis Research & Therapy 2008, 10:R19  doi:10.1186/ar2372

Published: 6 February 2008

Abstract

Background

Dietary flavonols may play an important role in the adjunct therapy of chronic inflammation. The availability of therapeutic formulations of pentahydroxyflavone glycoside, rutoside (RU), led us to investigate the ability of this molecule to modulate the release of various proinflammatory mediators from human activated macrophages in vitro and to ameliorate arthritic markers in a rat model.

Methods

RU was added simultaneously to human macrophages during their activation. Cells were then analyzed for inflammation-related gene expression using a specific array, and cell supernatants were collected to measure inflammatory mediators. RU was also injected into adjuvant-induced arthritic rats, and disease progression and body weight were evaluated until 50 days after injection. Sera and peritoneal macrophages were also collected to quantify the RU effect on various inflammatory markers.

Results

RU inhibited inflammation-related gene expression in activated human macrophages and the release of nitric oxide, tumor necrosis factor-alpha, interleukin (IL)-1, and IL-6 from these cells. In a rat model, RU inhibited clinical signs of chronic arthritis, correlating with decreased levels of inflammatory cytokines detected in rat sera and macrophage supernatants.

Conclusion

Thus, RU may have clinical value in reducing inflammatory manifestations in human arthritis and other inflammatory diseases.