Association of reduced heme oxygenase-1 with excessive Toll-like receptor 4 expression in peripheral blood mononuclear cells in Behçet's disease
1 Department of Internal Medicine and Clinical Immunology, Yokohama City University Graduate School of Medicine, 236-0004, 3-9 Fukuura, Kanazawa-ku, Yokohama, Japan
2 Department of Ophthalmology and Visual Science, Yokohama City University Graduate School of Medicine, 236-0004, 3-9 Fukuura, Kanazawa-ku, Yokohama, Japan
Arthritis Research & Therapy 2008, 10:R16 doi:10.1186/ar2367Published: 31 January 2008
Additional file 1:
The Protein and mRNA TLR2, TLR4 and HO-1 expression levels in PBMCs. (A) TLR2, TLR4, HO-1, and actin protein expression in PBMCs and CD14+/- cells from a healthy control individual (HC). (b) TLR2, TLR4, CD14, and β-actin mRNA expression levels in CD14+/- cells from HCs. (C) Correlation between densitometrically analyzed HO-1 protein levels and semiquantatively evaluated HO-1 mRNA expression by real-time PCR in PBMCs and CD14+/- cells from a HC.
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Additional file 2:
The correlation between HO-1 and TLR4 mRNA levels in PBMCs from patients with BD.
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Additional file 3:
The effect of LPS, PMB, and infliximab on TNF-α production by PBMCs. TNF-α levels in supernatants of PBMC-cultured media recovered after 24 hours of stimulation with LPS, with or without PMB and/or infliximab, as determined by ELISA.
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