Open Access Research article

Association of reduced heme oxygenase-1 with excessive Toll-like receptor 4 expression in peripheral blood mononuclear cells in Behçet's disease

Yohei Kirino1, Mitsuhiro Takeno1, Reikou Watanabe1, Shuji Murakami1, Masayoshi Kobayashi1, Haruko Ideguchi1, Atsushi Ihata1, Shigeru Ohno1, Atsuhisa Ueda1, Nobuhisa Mizuki2 and Yoshiaki Ishigatsubo1*

Author Affiliations

1 Department of Internal Medicine and Clinical Immunology, Yokohama City University Graduate School of Medicine, 236-0004, 3-9 Fukuura, Kanazawa-ku, Yokohama, Japan

2 Department of Ophthalmology and Visual Science, Yokohama City University Graduate School of Medicine, 236-0004, 3-9 Fukuura, Kanazawa-ku, Yokohama, Japan

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Arthritis Research & Therapy 2008, 10:R16  doi:10.1186/ar2367

Published: 31 January 2008

Additional files

Additional file 1:

The Protein and mRNA TLR2, TLR4 and HO-1 expression levels in PBMCs. (A) TLR2, TLR4, HO-1, and actin protein expression in PBMCs and CD14+/- cells from a healthy control individual (HC). (b) TLR2, TLR4, CD14, and β-actin mRNA expression levels in CD14+/- cells from HCs. (C) Correlation between densitometrically analyzed HO-1 protein levels and semiquantatively evaluated HO-1 mRNA expression by real-time PCR in PBMCs and CD14+/- cells from a HC.

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Additional file 2:

The correlation between HO-1 and TLR4 mRNA levels in PBMCs from patients with BD.

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Additional file 3:

The effect of LPS, PMB, and infliximab on TNF-α production by PBMCs. TNF-α levels in supernatants of PBMC-cultured media recovered after 24 hours of stimulation with LPS, with or without PMB and/or infliximab, as determined by ELISA.

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