Histological analyses for cartilage legions. (a) Representative histologies of the lateral femoral condyle and the lateral tibial plateau stained with safranin-O. Scale bar = 200 μm. (b) Influence of running 30 km on cartilage. Mankin's score of the right knee in rats after 30 km of strenuous running without hyaluronan injection was compared with that in control rats without strenuous running or hyaluronan injection. Data expressed as the mean ± standard deviation (n = 5). *P < 0.01, Mann–Whitney U test. (c) Effect of hyaluronan on cartilage. Mankin's score for cartilage legions. Rats were forced to run 30 km in 6 weeks. Hyaluronan was injected each week into the right knee. Nothing was injected into the left knee. Both sides of the knees were compared. Mankin's score expressed as the mean ± standard deviation (n = 5). **P < 0.05, Wilcoxon signed rank test.
Tang et al. Arthritis Research & Therapy 2008 10:R13 doi:10.1186/ar2363