This article is part of the supplement: Fourth International Synovitis Workshop

Meeting abstract

Evidence for an antigen-driven immune response in the chronically inflamed synovium

Claudia Bearek and H-J Kim

Author Affiliations

Deutsches Rheuma ForschungsZentrum, Berlin, Germany

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Arthritis Res 2000, 1(Suppl 1):S09-268 doi:10.1186/ar23


The electronic version of this article is the complete one and can be found online at: http://arthritis-research.com/15nov99/ar01s1


Published:15 November 1999

© 2000 Current Science Ltd

Full text

Recently it has been shown that proinflammatory cytokines, like TNF-alpha and lymphotoxin play a crucial role in the organogenesis of the lymphoid tissue. Thus, during chronic inflammation, in the affected tissue, these cytokines may promote the development of a micro-environment which supports a local immune response. In patients with rheumatoid arthritis large lymphocytic infiltrates are often seen in the synovial tissue. These cell clusters have a characteristic arrangement of T, B and plasma cells. Proliferating B cells are found only centrally in a network of follicular dendritic cells. Using micro-manipulation CD20+ B cells and plasma cells were isolated separately from different parts of single infiltrates. DNA was extracted and the VH/VL gene repertoires determined. The data show that within the network of follicular dendritic cells there is an oligoclonal expansion of B cells. During proliferation V-gene variants are generated by the hypermutation mechanism. The pattern of somatic mutations suggests that both naive and memory B cells become activated in the synovial tissue. Within single infiltrates we did not find identical rearrangements between CD20+ B and plasma cells. Nevertheless, the finding of clonally related plasma cells suggests that these cells underwent terminal differentiation in the synovial tissue. The analysis of individual B cells recovered from synovial tissue opens a new way to determine the specificity of those cells which take part in the local immune reaction.

References

  1. Berek C, Kim H-J: B-cell activation and development within chronically inflamed synovium in rheumatoid and reactive arthritis.

    Sem Immunol 1997, 9:261-268. Publisher Full Text OpenURL

  2. Fu Y-X, Chaplin DD: Development and maturation of secondary lymphoid organs.

    Annu Rev Immunol 1999, 17:399-433. PubMed Abstract | Publisher Full Text OpenURL

  3. Kim H-J, Krenn V, Steinhauser G, Berek C: Plasma cell development in synovial germinal centers in patients with rheumatoid and reactive arthritis.

    J Immunol 1999, 162:3053-3062. PubMed Abstract | Publisher Full Text OpenURL

  4. Randen I, Mellbye J, Forre O, Natvig JB: The identification of germinal centres and follicular dendritic networks in rheumatoid synovial tissue.

    Scand J Immuol 1995, 41:481-486. OpenURL

  5. Schröder AE, Greiner A, Seyfert C, Berek C: Differentiation of B cells in the nonlymphoid tissue of the synovial membrane of patients with rheumatoid arthritis.

    Proc Natl Acad Sci USA 1996, 93:221-225. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL